Vitamin c flush biofilm

Even as a young child, I knew the importance of taking vitamin C for your health.

vitamin c flush biofilm

Like many families we went through cycles of healthier eating, but vitamin C was always a staple regardless. Teaser alert, both vitamin C and magnesium, when the dosage exceeds your personal threshold, will cause frequent trips to the bathroom.

Lessons I learned the hard way, thanks Dad. In high doses, it becomes an ascorbic acid flush, a powerful cleansing agent, protecting the body from chemical toxins and infection.

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Like any detoxification program, afterwards the body feels refreshed and rejuvenated. The benefits of an ascorbic acid flush do not end there…. Each time a cleansing flush is done, the body needs less of the nutrient to reach its ideal level. Ascorbic acid is the primary preventative agent of free radical damage. It is needed for proper absorption of many minerals, such as iron and calcium. Without it, collagen, the connective tissue in skin, would be unable to form.

The Effects of Vitamin C in Sepsis and ARDS

Vitamin C also helps to prevent adrenal exhaustion by supporting the adrenal glands. Research has shown that people with high levels of ascorbic acid in the blood live longer than those with low levels. The benefits of an ascorbic acid flush are particularly valuable for those with influenza, allergies, or any chemical exposure. If diarrhea occurs again, lower the dosage. To optimize the Vitamin C Flush, start in the morning on an empty stomach. Expect to cleanse for two to four hours, although for some people, more time is needed.

The healthier your body is, the less vitamin C you will require. Stop and wait or slow down. Sometimes if you continue on you will experience prolonged flushing.M y wife joined me at the breakfast table and saw my bloodshot eyes streaming tears down my face. One eye was partially closed with the lids swollen — behind that eye was a migraine that started 36 hours before when I began my liver flush. My body ached as if I had the flu and I had the feeling my body was in crisis.

And that is the truth — I had used both charcoal and DMSA to soak up some of the toxins I knew were going to come out. At first I was disappointed when this liver flush yielded no stones. But then around 10 AM I started passing the yellow gelatinous stuff, larger thicker blobs than I had seen before. Then, in the afternoon my abdomen began cramping, and I started feeling body aches and general malaise. A few hours later, with the help of an enema, I started passing large quantities of what I previously called mucoid plaque.

It might be a toxic stew of pathogens which could include bacteria, virus and fungi probably marinated in heavy metals. The biofilm matrix has a horizontal and a vertical weave. It is standard knowledge that biofilm bacteria use metals and minerals to help build that matrix. I decided to take better pictures this time including close-ups. My colonic may have helped also. My migraine disappeared this morning after I delivered the last bits of slime and I was left feeling like I had exorcised a demon.

No amount of vitamins or chelation could restore my health with these toxic demons choking up my insides. Steve calls it cyanobacteria. Flat, filmlike slime above, hard to get a good photograph because it punches together easily and sinks to the bottom of the container. Probably more cyanobacteria. To give you a sense of scale, the next images are taken on a paper plate. Steve says these are Bryophyte materials related to mosses, liverworts and hornworts.

Maybe tough enough to win the war against your biofilm…. A word of caution with any type of medical implants. Biofilm and bacteria infections are associated with all medical implants. Biofilm is the reason for antibiotic resistant bacteria and recurring infections.

Dr. Ettinger’s Biofilm Protocol for Lyme and Gut Pathogens

Biofilm is a slime that forms around medical devices and allows mycotoxins such as mold and fungus to remain undectected.Many of us are not eating enough Vitamin C-rich foods and sadly, our food supply contains less and less Vitamin C because of premature food harvesting, artificial ripening, and food processing. Nobel Laureate Dr. Linus Pauling was perhaps the best known advocate of the benefits of Vitamin C.

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Vitamin C is one of the most important vitamins your body requires and the only way to get Vitamin C is through diet. Animals, when adjusted for human size and weight, make the equivalent of grams of Vitamin C a day, mostly in their livers when stress free.

Production can more than double when the animal is distressed but so too is consumption by the body! Our genetic ancestors actually once had the ability to synthesise Vitamin C but lost it millions of years ago.

Scientists estimate that without this mutation we would be making grams of Vitamin C a day this is 10,mg.

This makes the RDA of just 45mg look horribly inadequate. For this reason it is important you choose a day to do the flush when you can remain at home, near the bathroom.

As your health improves you may notice that, each time you do the Vitamin C Flush, you need less total Vitamin C to reach bowel tolerance. A severe cold can increase tolerance to grams; an influenza, even up to grams; and mononucleosis or viral pneumonias, to as much as grams per 24 hours.

vitamin c flush biofilm

Any vitamin C that is unable to be absorbed is simply passed out through the bowel. However, there are some people for whom a vitamin C flush is not advised:. Thanks for the tips Amie! Perfect timing x. Hi I have swolen lymph nodes and was wondering if it was safe to do the flush I have them in the groin and I feel it is continuing to spread. There are a few things that may be causing the swelling in your lymph nodes and it is very important that you have that medically assessed as soon as possible.

If the swelling is a secondary result of an infection, a vitamin C flush amongst other support, not just on its ownwill be helpful. So this weekend we will all be doing the flush x. I hope the flush is going well and that you all bounce back soon.

vitamin c flush biofilm

You might also want to consider some Zinc and Vitamin D too. Wishing you all a speedy recovery X. Thank you Amie for this article. Can you please explain why? Thank you! Having already been diagnosed with Gilbert Syndrome, optimal vitamin c levels will only be useful for you! I started the flush early this morning at I did not have diariea until about pm tonight. And before the flood gates opened.

I was told to keep taking it every 15 minutes until clear watery stooka. I have so far taken 36 tsp. What does that come to for tomorrow.The plaque that forms on your teeth and causes decay is one type of bacterial biofilm.

If you have ever walked in a stream or river, you may have slipped on biofilm-coated rocks. Biofilms significantly increase bacterial resistance to antibiotics and innate host defenses.

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Biofilm not only lives on surfaces in nature, populations of these microorganisms are in your gut and that of your pet. Biofilm microorganisms are of two types: free-living planktonic microbes and colonizing sessile biofilm organisms. They produce a matrix that encases them so they strongly adhere to surfaces and resist dislodgement. The biofilm of healthful commensal microorganisms greatly contributes to intestinal barrier function and colonization resistance.

Disrupted, healthful biofilm permits colonization and biofilm formation by potential pathogens such as Klebsiella pneumonia, E. Microbes residing within biofilms may consist of one or more species that communicate and collaborate with one another in a heterogeneous community.

Biofilm formation appears to be initiated by contact with a surface. It is a survival mechanism that triggers regulation of genes such as those mediating motility and growth as well as expression of genes regulating synthesis of exopolysaccharides and exoproteins. The presence of calcium, iron, and magnesium is essential for biofilm creation and serves to cross-link the anionic regions of polymers.

Life within a biofilm provides significant survival advantages to bacteria and yeasts. Biofilms adhere to surfaces and resist dislodgement. Biofilm formation may be viewed as an adaptive mechanism that enables microbes to persist and reproduce in an advantageous micro ecological niche.

They are protected from predation by phages and protozoa as well as from host immune responses and are highly resistant to the spectrum of antimicrobials ranging from germicides and disinfectants to antibiotics and bacteriocins produced by probiotics. Sessile biofilm microorganisms are 10 to times more resistant to antibiotics compared to planktonic forms of the same strain.

It is believed that antimicrobial resistance to biofilm-associated organisms is due to impaired ability to penetrate the biofilm matrix, reduced microbial growth rates, biofilm-induced expression of resistance factors, and other biofilm-associated physiologic changes that decrease the susceptibility to antimicrobials.

Eradication of pathogen-associated biofilm is critical to successful elimination of these harmful pathogens and restoration of healthful biofilm communities.

John B. See our FAQ for more detailed days and hours.

Vitamin C Flush

Location and Hours John B.Extracellular polymeric substances EPS produced by bacteria form a matrix supporting the complex three-dimensional architecture of biofilms. In addition to supporting the community structure, the EPS matrix protects bacterial biofilms from the environment. Specifically, it shields the bacterial cells inside the biofilm, by preventing antimicrobial agents from getting in contact with them, thereby reducing their killing effect.

New strategies for disrupting the formation of the EPS matrix can therefore lead to a more efficient use of existing antimicrobials. Here we examined the mechanism of the known effect of vitamin C sodium ascorbate on enhancing the activity of various antibacterial agents. Our quantitative proteomics analysis shows that non-lethal concentrations of vitamin C inhibit bacterial quorum sensing and other regulatory mechanisms underpinning biofilm development.

As a result, the EPS biosynthesis in reduced, and especially the polysaccharide component of the matrix is depleted. Once the EPS content is reduced beyond a critical point, bacterial cells get fully exposed to the medium. At this stage, the cells are more susceptible to killing, either by vitamin C-induced oxidative stress as reported here, or by other antimicrobials or treatments.

Bacterial biofilms are culprits of various human infectious diseases, industrial corrosion and food contamination Flemming et al. Bacteria within the biofilms synthesize a dense protective matrix composed of extracellular polymeric substances EPS Branda et al. Exopolysaccharides and proteins are the most abundant component of the biofilm matrix, defining its physico-chemical properties and morphology Marvasi et al.

Furthermore, the EPS serve as a food storage, which gets mobilized during extended nutrient depletion Xiao et al.

The structure of the EPS matrix varies considerably among bacterial strains, and its composition is influenced by the local environment and nutrient availability. Antibiotics are widely used to eradicate bacterial biofilms when treating infections.

However, their prolonged use increases the risk of developing multi-resistant strains, and disrupts the ecology of the residential microflora Cegelski et al. Hence an increasing interest for chemo-prophylactic agents, which can affect biofilm formation, and thereby reduce the time and dose of antibiotics treatments Xavier et al.

Vitamin C, a major dietary micronutrient, has been shown to exhibit bactericidal activity against mycobacteria Vilcheze et al. However, this killing effect seems to be confined to mycobacteria, since vitamin C did not kill other opportunistic bacterial pathogens, such as Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coliand Pseudomonas aeruginosa Khameneh et al.

Interestingly, vitamin C has been reported to enhance the effect of antibiotics vs. Similarly, vitamin C has been shown to enhance the killing effect of a physical bactericidal agent, cold atmospheric plasma, against biofilms of S. In this study we set out to characterize the mechanism of this non-lethal synergistic effect of vitamin C, which enhances the effect of antibiotics and physical killing agents.

We performed an initial characterization with several bacterial strains, and different doses of vitamin C. Our conclusion was that while the low doses of vitamin C are harmless to the planktonic bacteria, they effectively destabilizes biofilms. We then focused on an in-depth quantitative analysis with Bacillus subtilisa model organism for biofilm development Vlamakis et al.

Our findings, based on quantification of the biofilm EPS content and cell viability, quantitative proteome analyses and genome-scale metabolic modeling point to a vitamin C-dependent inhibition of the synthesis of polysaccharides that form the biofilm matrix. This proceeds via inhibition of the quorum sensing and other regulatory mechanisms, leading to repression of specific biosynthetic operons.

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Once the EPS content is reduced beyond a critical point, bacterial cells become exposed, and more susceptible to killing by any external factors. LB 10 g of tryptone, 5 g of yeast extract and 5 g of NaCl per liter or solid LB medium supplemented with 1.

Sodium ascorbate was purchased from Sigma—Aldrich. For the biofilm analysis, 24 h old biofilms, grown in the presence of various concentrations of sodium ascorbate, were removed and sonicated at 10 W for 30 s to homogenize the biofilm.

Briefly, for the determination of biomass, the homogenized suspension was washed three times g for 20 min with sterile water, lyophilized and weighed.

The supernatant from the homogenized suspensions were collected after centrifugation g20 min and protein content was quantified by using the Bradford assay. The effect of sodium ascorbate on B. The toxicity of these fluorescence probe toward the bacterial cells in biofilms was evaluated by comparison of viability and biomass with control samples. After 24 h, the biofilms were exposed to 2. The stained biofilms were transferred to a glass slide and laser scanning confocal microscope imaging of the biofilms was performed using an LSM NLO Carl Zeiss equipped with argon-ion and helium neon lasers.

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For the detection of reactive oxygen species ROS in biofilm cells, 24 h old B. The stained biofilms were visualized by fluorescence microscopy. Imaging was performed with a fluorescence microscope Axio Imager 2.I want to help you and I can create a tailor-made protocol for your individual situation. Please fill out the distance patient application link above. If you have a specific question about the program please call me first at Ettinger, DC A specific question has been asked a lot lately, as to what is my protocol for busting through bacterial biofilm.

This question has mostly come from people diagnosed with or those who think they may have H. MacDonald, MD. I hope the data below is helpful to you in your search for information or health restoration. Figure 1: The life cycle of biofilm. Dental plaque, the slimy coating that fouls pipes and tanks, and algal mats on bodies of water are examples of biofilms. While biofilms are generally pathogenic in the body, causing such diseases as cystic fibrosis and otitis media, they can be used beneficially in treating sewage, industrial waste, and contaminated soil.

Therefore, these sessile organisms predominate in most of the environmental, industrial, and medical problems and processes of interest to microbiologists. If biofilm bacteria were simply planktonic cells that had adhered to a surface, this revelation would be unimportant, but they are demonstrably and profoundly different.

vitamin c flush biofilm

We first noted that biofilm cells are at least times more resistant to antibacterial agents. Now we have discovered that adhesion triggers the expression of a sigma factor that derepresses a large number of genes so that biofilm cells are clearly phenotypically distinct from their planktonic counterparts.

Each biofilm bacterium lives in a customized microniche in a complex microbial community that has primitive homeostasis, a primitive circulatory system, and metabolic cooperativity, and each of these sessile cells reacts to its special environment so that it differs fundamentally from a planktonic cell of the same species.

Lappin-Scott HM, et. Microbial biofilms. Annu Rev Microbiol. A simulation of the bacteria Vibrio cholerae forming a biofilm, with each slightly curved, rod-shaped unit indicating individual bacteria. Excerpts from a Klaire Labs, product monograph, which is a basic primer on the topic of bacterial biofilm.

Biofilm thus renders pathogenic microorganisms enormously difficult to eradicateand can almost single-handedly contribute to localized or systemic inflammatory reactions, autoimmune reactions, and delayed wound healing. Once established, however, biofilm infections persist.

They are rarely resolved by host defense mechanisms, even in individuals with healthy innate and adaptive immune reactions.

Dr. Ettinger’s Biofilm Protocol for Lyme and Gut Pathogens

Active host responses, such as invading neutrophils the most abundant type of white blood cell in mammals and forms an essential part of the innate inborn; natural immune systemcan even be detrimental since those cells can cause collateral damage to neighboring healthy host tissue.

Biofilm infections respond only transiently to antibiotic therapy. This is why Interfase Plus and lactoferrin are so useful. Here is a good video on Chlamydia and biofilm Video [biofilm section min. Wilmore Webley on C.No dl-ascorbate or d-ascorbate should be used as the d-ascorbate form is not absorbed by humans; people take up only the l-ascorbate. Per gram of ascorbate, we find best outcomes, patient compliance, and satisfaction from a balanced mineral content of potassium 66 mg.

If there is less than 1. When possible, it is best to start especially the first use of this protocol on an empty stomach, first thing in the morning. Most people saturate their ascorbate need within a few hours. Dissolve each half-teaspoon 1. Plan to count and record each dosage.

After dissolving the l-ascorbate and allowing any effervescence to abate typically dissolves within two minutesdrink the beverage. The amount of l-ascorbate needed depends on how quickly your body uses it up.

Below are suggestions for how to best determine your needs based on how healthy you are:. Continue with these instructions at the proper time intervals until you reach a watery stool or an enema-like evacuation of liquid from the rectum. This is as if a quart or so of liquid is expressed from the rectum. At this time, stop consuming the buffered ascorbate for the day. Example: 30 grams 10 teaspoons may be dissolved in ounces of liquid. If this method is chosen, we recommend using a capped, dark bottle to avoid air or light photo- oxidation of the ascorbate.

Dissolved ascorbate is stable for a day if kept cool or cold and tightly sealed. As you become healthier, the ascorbate is used more efficiently and is better conserved in your body and less ascorbate will be needed to achieve the desired effect.

As your need for ascorbate decreases, you may notice loosening of the stool indicating that your body is consuming ascorbate more efficiently and your need has decreased. That is the time to taper ascorbate intake. You may use ascorbate as a liquid, tablet, or a capsule taking two to four or mor e doses per day. This is the current daily sufficiency need for ascorbate. Many people report a subjective sense of improved well-being after the completion of an ascorbate calibration.

This may be of short duration, initially, but is a promising sign for long-term improvement.


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